Further characterization of the Krebs tricarboxylic acid cycle metabolon.

Is there tight channelling in the tricarboxylic acid cycle metabolon?

Is there tight channelling in the tricarboxylic acid cycle metabolon? Balazs Sumegi,§ A. Dean Sherry,§ Craig R. Malloy,*$ Claudia Evans*t and Paul A. Srere*t *Pre-Clinical Science Unit of the Department of Veterans Affairs Medical Center, tDepartment of Biochemistry and $The Mary Nell & Ralph B. Rogers N.M.R. Center, The University of Texas Southwestern Medical Center at Dallas, 4500 South Lanc...

Malonate inhibition of oxidations in the Krebs tricarboxylic acid cycle.

The inhibition of succinate oxidation by malonate is a well known phenomenon. Since the oxidation of succinate to fumarate is an integral part of the Krebs cycle of oxidations, it has been generally assumed that the inhibitory effect of malonate upon the oxidation of any member of the cycle is the result of the inhibition of the succinate to fumarate step. However, the present paper provides ev...

Tricarboxylic Acid Cycle in

The Tricarboxylic Acid Cycle in Dictyostelium discoideum

Some aspects of tricarboxylic acid-cycle activity during differentiation and aging in Dictyostelium discoideum were examined. The concentrations of glutamate, aspartate, alanine, citrate, 2-oxoglutarate, succinate, fumarate, malate, oxaloacetate, pyruvate and acetyl-CoA were determined at four stages over the course of differentiation. The rate of 02 utilization was also determined over differe...

The Tricarboxylic Acid Cycle in Dictyostelium discoideum

Experimental studies of enzyme kinetics in vitro and metabolic fluxes in vivo have been used by Wright and her colleagues to develop a detailed kinetic model of the tricarboxylic acid cycle in Dictyostelium discoideum. This model has recently been been analyzed by two different methods (Albe, K. R., and Wright, B. E. (1992) J. Biol. Chem. 267, 3106-3114; Shiraishi, F., and Savageau, M. A. (199...